Summary
The homeostasis of fluid bathing the luminal surface of the vocal folds is important
for phonation and laryngeal defense. Dehydration of the respiratory tract during mouth
breathing can perturb the concentration of sodium and chloride ions in surface fluid.
Exposure to dry air also increases the osmolarity of airway surface fluid. We hypothesized
that viable vocal fold epithelium would detect changes in the ionic and osmotic composition
of fluid on the luminal surface. Therefore, we examined bioelectric responses of vocal
folds exposed to physiologically real, luminal ionic and osmotic challenges in vitro. The study used randomized factorial design with experimental and sham control groups.
Fifty native ovine vocal folds were exposed to five challenges (ionic, osmotic, combined
ionic-osmotic, and sham) on the luminal surface. Bioelectric measures of potential
difference (PD), short-circuit current (ISC), and tissue resistance were assessed at prechallenge baseline, during challenge,
and after removal of challenge. Ionic and combined ionic-osmotic challenges reduced
PD and ISC (P<0.01). These reductions depended on the nature of the ionic challenge, were observed
within 10 minutes, lasted for the duration of exposure, and were reversible after
removal of the challenge. Conversely, sham or osmotic challenge did not alter bioelectric
parameters over time (P>0.05). Viable ovine vocal fold epithelia detect ionic perturbations to the luminal
surface. This sensitivity to luminal ionic challenge may be necessary to maintain
the homeostasis of surface fluid.
Key Words
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Article Info
Publication History
Published online: February 07, 2007
Accepted:
November 20,
2006
Footnotes
This work was presented at the 34th Annual Symposium of the Voice Foundation: Care of the Professional Voice, Philadelphia, Pennsylvania, June 1, 2005.
Identification
Copyright
© 2008 The Voice Foundation. Published by Elsevier Inc. All rights reserved.
