Normalization Patterns of the Surface Electromyographic Signal in the Phonation Evaluation



      The aim of the study was to compare the different parameters, regarding the peak and mean, at different phonatory tasks for standardization of electromyography signal of electrical activity (EA) of the laryngeal extrinsic muscles on voice evaluation.


      The electrical potentials of the suprahyoid (SH) and infrahyoid (IH) muscles of 35 voluntary nondysphonic subjects were evaluated using three evaluations of rest, two maneuvers to determine maximum voluntary sustained activity (MVSA), and usual and strong intensity of vowel /ɛ/ and 20–30 count emissions. The EA signal was converted using root mean square in microvolts and normalized by mean and peak of each task. The selected normalization task was that with minor coefficient of variation for all muscles.


      The tasks that provided minor coefficient of variation of EA in both muscle groups were the peak of vowel /ɛ/ (mean potentials equal to 43.31 ± 2.97 for right IH, 36.27 ± 2.76 for left IH, and 42.11 ± 2.57 for SH) and the 20–30 count emissions (mean potentials equal to 31.30 ± 308 for right IH, 30.56 ± 2.76 for left IH, and 30.43 ± 4.22 for SH), both in usual intensity and MVSA, as second option.


      The peak of vowel /ɛ/ and 20–30 count emissions is usual intensity, and the MVSA as second option should be considered for signal normalization in SH and IH muscles, and may provide conditions for using the surface electromyography in voice clinic.

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        • Burnett A.F.
        • Green J.
        • Netto K.
        • Rodrigues J.
        Examination of EMG normalization methods for study of the posterior and posterolateral neck muscles in healthy controls.
        J Electromyogr Kinesiol. 2007; 17: 635-641
        • Netto K.J.
        • Burnett A.F.
        Reliability of normalisation methods for EMG analysis of neck muscles.
        Work. 2006; 26: 123-130
      1. Marchetti PH, Duarte M. Instrumentação em Eletromiografia. 2006. Available at: Accessed November 20, 2010.

        • Knutson L.M.
        • Soderberg G.L.
        • Ballantyne B.T.
        • Clarke W.R.
        A study of various normalization procedures for within day electromyographic data.
        J Electromyogr Kinesiol. 1994; 4: 47-59
        • Stepp C.E.
        Surface electromyography for speech and swallowing systems: measurement, analysis, and interpretation.
        J Speech Lang Hear Res. 2012; 55: 1232-1246
        • Balata P.M.M.
        • Silva H.J.
        • Nascimento G.K.B.O.
        • et al.
        Incomplete swallowing and retracted tongue maneuvers for electromyographic signal normalization of the extrinsic muscles of the larynx.
        J Voice. 2012; 26: 813.e1-813.e7
        • Stepp C.E.
        • Heaton J.T.
        • Stadelman-Cohen T.K.
        • Braden M.N.
        • Jetté M.E.
        • Hillman R.E.
        Characteristics of phonatory function in singers and nonsingers with vocal fold nodules.
        J Voice. 2011; 25: 714-724
        • Stepp C.E.
        • Hillman R.E.
        • Heaton J.T.
        Modulation of neck intermuscular Beta coherence during voice and speech production.
        J Speech Lang Hear Res. 2011; 54: 836-844
        • Stepp C.E.
        • Heaton J.T.
        • Rolland R.G.
        • Hillman R.E.
        Neck and face surface electromyography for prosthetic voice control after total laryngectomy.
        IEEE Trans Neural Syst Rehabil Eng. 2009; 17: 146-155
        • Stepp C.E.
        • Heaton J.T.
        • Jetté M.E.
        • Burns J.A.
        • Hillman R.E.
        Neck surface electromyography as a measure of vocal hyperfunction before and after injection laryngoplasty.
        Ann Otol Rhinol Laryngol. 2010; 119: 594-601
        • Pettersen V.
        • Bjorkoy K.
        • Torp H.
        • Westgaard R.H.
        Neck and shoulder muscle activity and thorax movement in singing and speaking tasks with variation in vocal loudness and pitch.
        J Voice. 2005; 19: 623-634
        • Pettersen V.
        • Westgaard R.H.
        The activity patterns of neck muscles in professional classical singing.
        J Voice. 2005; 19: 238-251
        • Loucks T.M.J.
        • Poletto C.J.
        • Saxon K.G.
        • Ludlow C.L.
        Laryngeal muscle responses to mechanical displacement of the thyroid cartilage in humans.
        J Appl Physiol. 2005; 99: 922-930
        • Heaton J.T.
        • Goldstein E.A.
        • Kobler J.B.
        • et al.
        Surface electromyographic activity in total laryngectomy patients following laryngeal nerve transfer to neck strap muscles.
        Ann Otol Rhinol Laryngol. 2004; 113: 754-764
        • Silvério K.C.A.
        Atividade elétrica dos músculos esternocleidomastóideo e trapézio fibras superiores em indivíduos normais e disfônicos.
        ([Dissertação]) Universidade Estadual de Campinas, São Paulo, Brazil1999
        • Redenbaugh M.A.
        • Reich A.R.
        Surface EMG and related measures in normal and vocally hyperfunctional speakers.
        J Speech Hear Disord. 1989; 54: 68-73
      2. Yamasaki R, Leão SHS, Madazio G, Padovani M, Azevedo R, Behlau M. Correspondência entre Escala Analógico-Visual e a Escala Numérica na avaliação perceptivo-auditiva de vozes. In: XVI Congresso Brasileiro de Fonoaudiologia. 2008; Campos de Jordão, SP, Brazil.

        • Whitley E.
        • Ball J.
        Statistics review 4: sample size calculations.
        Crit Care. 2002; 6: 335-341
        • IBGE. Instituto Brasileiro de Geografia e Estatística
        Normas de apresentação tabular.
        3rd ed. IBGE, Rio de Janeiro, Brazil1993
        • Coriolano W.S.
        • Belo L.R.
        • Carneiro D.
        • et al.
        Swallowing in patients with Parkinson's disease: a surface electromyography study.
        Dysphagia. 2012; 27: 550-555
        • Pernambuco L.A.
        • Silva H.J.
        • Lima L.M.
        • et al.
        Electrical activity of masseter muscle in young adults during swallowing of liquid.
        J Soc Bras Fonoaudiol. 2011; 23: 214-219
        • Yeates E.M.
        • Steele C.M.
        • Pelletier C.A.
        Tongue pressure and submental surface electromyography measures during noneffortful and effortful saliva swallows in healthy women.
        Am J Speech Lang Pathol. 2010; 19: 274-281
        • Nagae M.H.
        • Alves M.C.
        Electromyographic study of swallowing in suprahyoid musculature in Angle's Class I and III subjects..
        Rev CEFAC. 2009; 11: 355-362
        • Huckabee M.L.
        • Steele C.M.
        An analysis of lingual contribution to submental surface electromyographic measures and pharyngeal pressure during effortful swallow.
        Arch Phys Med Rehabil. 2006; 87: 1067-1072
        • Palmer P.M.
        • Jaffe D.M.
        • McCulloch T.M.
        • Finnegan E.M.
        • Van Daele D.J.
        • Luschei E.S.
        Quantitative contributions of the muscles of the tongue, floor-of-mouth, jaw, and velum to tongue-to-palate pressure generation.
        J Speech Lang Hear Res. 2008; 51: 828-835
        • Ding R.
        • Larson C.R.
        • Logemann J.A.
        • Rademaker A.W.
        Surface electromyographic and electroglottographic studies in normal subjects under two swallow conditions: normal and during the Mendelsohn manuever.
        Dysphagia. 2002; 17: 1-12
        • Balata P.M.M.
        • Silva H.J.
        • Moraes K.J.R.
        • Pernambuco L.A.
        • Moraes S.R.A.
        The use of surface electromyography in phonation studies: an integrative review.
        Int Arch Otorhinolaryngol. 2013; 17: 329-339
        • Sapir S.
        • Larson K.K.
        Supralaryngeal muscle activity during sustained vibrato in four sopranos: surface EMG findings.
        J Voice. 1993; 7: 213-218
        • Lapatki B.G.
        • Oostenveld R.
        • Van Dijk J.P.
        • Jonas I.E.
        • Zwarts M.J.
        • Stegeman D.F.
        Optimal placement of bipolar surface EMG electrodes in the face based on single motor unit analysis.
        Psychophysiology. 2010; 47: 299-314
        • Milutinović Z.
        • Lastovka M.
        • Vohradník M.
        • Janosević S.
        EMG study of hyperkinetic phonation using surface electrodes.
        Folia Phoniatr (Basel). 1988; 40: 21-30
        • Hocevar-Boltezar I.
        • Janko M.
        • Zargi M.
        Role of surface EMG in diagnostics and treatment of muscle tension dysphonia.
        Acta Otolaryngol. 1998; 118: 739-743
        • Sapir S.
        • Baker K.K.
        • Larson C.R.
        • Ramig L.O.
        Short-latency changes in voice F0 and neck surface EMG induced by mechanical perturbations of the larynx during sustained vowel phonation.
        J Speech Lang Hear Res. 2000; 43: 268-276