Research Article| Volume 31, ISSUE 4, P504.e35-504.e40, July 2017

Human Papillomavirus Infection Status of Various Laryngeal Diseases in Japan: A Comprehensive Study

Published:December 13, 2016DOI:



      The aim of our study was to clarify the human papillomavirus (HPV) infection status of various laryngeal diseases in Tokyo, Japan.

      Study Design

      This is a retrospective study.


      A total of 144 patients who underwent surgical resection for various laryngeal lesions were enrolled in this study. These subjects were categorized into four groups based on lesion type: non-neoplastic, 44; precancerous, 29; cancer, 35; and papilloma, 36. To determine the rate of HPV infection, laryngeal secretions and resected tissue from our study participants were examined by liquid-phase hybridization (LPH) and consensus primer-directed polymerase chain reaction (PCR).


      The LPH for low-risk HPV was applied to all 144 patients, and that for high-risk HPV was additionally applied to 121 of the 144 patients. The PCR was applied to 94 of the 144 patients. The LPH detected low-risk HPV-DNA in 23 patients (1 cancer and 22 papillomas) and high-risk HPV-DNA in 3 patients (1 cancer and 2 papillomas). The PCR detected HPV-6 and HPV-11 in the papilloma group, whereas it detected HPV-31 in one patient with laryngeal cancer and one patient with precancerous lesion. Both the LPH and the PCR revealed the HPV infection rate in the non-neoplastic group to be 0%.


      Although we found no significant difference in the HPV-DNA positive rates of laryngeal cancer and precancerous lesions in the non-neoplastic group, the positive rates were significantly smaller in this group than in the papilloma group. In the Tokyo area, HPV had little or no association with laryngeal cancer, precancerous lesions, and non-neoplastic lesions in the larynx.

      Key Words

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        • Daling J.R.
        • Madeleine M.M.
        • Johnson L.G.
        • et al.
        Human papillomavirus, smoking, and sexual practices in the etiology of anal cancer.
        Cancer. 2004; 101: 270-280
        • Schiffman M.
        • Castle P.E.
        • Jeronimo J.
        • et al.
        Human papillomavirus and cervical cancer.
        Lancet. 2007; 370: 890-907
        • Pytynia K.B.
        • Dahlstrom K.R.
        • Sturgis E.M.
        Epidemiology of HPV-associated oropharyngeal cancer.
        Oral Oncol. 2014; 50: 380-386
        • Japan Health Promotion & Fitness Foundation
        Adult smoking rate.
        (Japan Health Promotion & Fitness Foundation web; In Japanese; Available at:) (Accessed July 31, 2016)
        • Katanoda K.
        • Matsuda T.
        • Matsuda A.
        • et al.
        An updated report of the trends in cancer incidence and mortality in Japan.
        Jpn J Clin Oncol. 2013; 43: 492-507
        • Makiyama K.
        • Hirai R.
        • Matsuzaki H.
        • et al.
        Assessment of human papilloma virus infection in adult laryngeal papilloma using a screening test.
        J Voice. 2013; 27: 230-235
        • Li X.
        • Gao L.
        • Li H.
        • et al.
        Human papillomavirus infection and laryngeal cancer risk: a systematic review and meta-analysis.
        J Infect Dis. 2013; 207: 479-488
        • Clavel C.
        • Masure M.
        • Bory J.-P.
        • et al.
        Hybrid Capture II-based human papillomavirus detection, a sensitive test to detect in routine high-grade cervical lesions: a preliminary study on 1518 women.
        Br J Cancer. 1999; 80: 1306-1311
        • Clavel C.
        • Masure M.
        • Bory J.-P.
        • et al.
        Human papillomavirus testing in primary screening for the detection of high-grade cervical lesions: a study of 7932 women.
        Br J Cancer. 2001; 84: 1616-1623
        • Ronco G.
        • Giorgi-Rossi P.
        • Carozzi F.
        • et al.
        Human papillomavirus testing and liquid-based cytology in primary screening of women younger than 35 years: results at recruitment for a randomised controlled trial.
        Lancet Oncol. 2006; 7: 547-555
        • Yoshikawa H.
        • Kawana T.
        • Kitagawa K.
        • et al.
        Detection and typing of multiple genital human papillomaviruses by DNA amplification with consensus primers.
        Cancer Sci. 1911; 82: 524-531
        • Kanda Y.
        Investigation of the freely available easy-to-use software “EZR” for medical statistics.
        Bone Marrow Transplant. 2013; 48: 452-458
        • Duray A.
        • Descamps G.
        • Arafa M.
        • et al.
        High incidence of high-risk HPV in benign and malignant lesions of the larynx.
        Int J Oncol. 2011; 39: 51-59
        • Chitose S.
        • Sakazaki T.
        • Ono T.
        • et al.
        Immunological responses against human papilloma virus and human papilloma virus induced laryngeal cancer.
        J Laryngol Otol. 2010; 124: 659-662
        • Lee L.-A.
        • Cheng A.-J.
        • Fang T.-J.
        • et al.
        High incidence of malignant transformation of laryngeal papilloma in Taiwan.
        Laryngoscope. 2008; 118: 50-55
        • Mehanna H.
        • Franklin N.
        • Compton N.
        • et al.
        Geographic variation in human papillomavirus-related oropharyngeal cancer: data from 4 multinational randomized trials.
        Head Neck. 2016; 38: E1863-E1869
        • Deng Z.
        • Hasegawa M.
        • Matayoshi S.
        • et al.
        Prevalence and clinical features of human papillomavirus in head and neck squamous cell carcinoma in Okinawa, southern Japan.
        Eur Arch Otorhinolaryngol. 2011; 268: 1625-1631
        • Gheit T.
        • Abedi-Ardekani B.
        • Carreira C.
        • et al.
        Comprehensive analysis of HPV expression in laryngeal squamous cell carcinoma.
        J Med Virol. 2014; 86: 642-646
        • Laskaris S.
        • Sengas I.
        • Maragoudakis P.
        • et al.
        Prevalence of human papillomavirus infection in Greek patients with squamous cell carcinoma of the larynx.
        Anticancer Res. 2014; 34: 5749-5753
        • Atighechi S.
        • Meybodian M.
        • Dadgarnia M.H.
        • et al.
        Investigating the prevalence of human papilloma virus in squamous cell carcinoma of the larynx and its correlation with disease prognosis.
        Iran J Otorhinolaryngol. 2016; 28: 197-202