Summary
Objective
The diffusion characteristics of water molecules were measured in the vocal folds
of canines exhibiting unilateral vocal fold paralysis and unilateral cricoarytenoid
joint dislocation. These characteristics were used in conjunction with a histological
examination of the microstructural changes of vocal fold muscle fibers to explore
the feasibility of diffusion tensor imaging (DTI) in distinguishing unilateral vocal
fold paralysis and unilateral cricoarytenoid joint dislocation as well as evaluating
microstructural changes.
Methods
Ten beagles were randomly divided into three groups: four in the unilateral vocal
fold paralysis group, four in the unilateral cricoarytenoid joint dislocation group,
and two in the normal group. Unilateral recurrent laryngeal nerve resection was performed
in the vocal fold paralysis group. Unilateral cricoarytenoid joint dislocation surgery
was performed in the dislocation group. No intervention was performed in the normal
group. Four months postintervention, the larynges were excised and put into a magnetic
resonance imaging (MRI) system (9.4T BioSpec MRI, Bruker, German) for scanning, followed
by an analysis of diffusion parameters among the different groups for statistical
significance. After MRI scanning, the vocal folds were cut into sections, stained
with hematoxylin and eosin, and scanned digitally. The mean cross-sectional area of
muscle fibers, and the mean diameter of muscle fibers in the vocal folds were calculated
by target detection and extraction technology. Mean values of each measurement were
used to compare the differences among the three groups. Pearson correlation analysis
was performed on the DTI parameters and the results from histological section extraction.
Results
The paralysis group had significantly higher Fractional Anisotropy (FA) compared to
the dislocation group and normal group (P = 0.004). The paralysis group also had a significantly lower Tensor Trace value compared
to the dislocation group and normal group (P = 0.000). The average cross-sectional area of vocal fold muscle fibers in the paralysis
group was significantly smaller than the dislocation group and normal group (P = 0.000). Pearson correlation analysis yielded values of, r = -0.785, P = 0.01 between the average cross-sectional area of vocal muscle fibers and FA, and
values of r = 0.881, P = 0.00 between Tensor Trace and the average cross-sectional area of vocal muscle.
Conclusion
FA and Tensor Trace can be used as effective parameters to reflect the changes of
microstructure in vocal fold paralysis and cricoarytenoid joint dislocation. DTI is
an objective and quantitative method to effectively evaluate unilateral vocal fold
paralysis and unilateral cricoarytenoid joint dislocation, also capable of noninvasively
evaluating vocal fold muscle fiber microstructure.
Key words
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Article info
Publication history
Published online: December 31, 2020
Accepted:
December 10,
2020
Footnotes
Financial disclosure: This study was supported by the National Natural Science Foundation of China, NSFC81970871.
Identification
Copyright
© 2020 The Voice Foundation. Published by Elsevier Inc. All rights reserved.
