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Letter| Volume 37, ISSUE 3, P458-460, May 2023

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Author response to Microstimulation in the Periaqueductal Gray Matter

      We have repudiated neither the methods nor the conclusions of the research undertaken by Dr. Pamela Davis and Dr. Shi Ping Zang. On the contrary we have duly cited their major works.
      • Zhang SP
      • Davis PJ
      • Bandler R
      • et al.
      Brain stem integration of vocalization: role of the midbrain periaqueductal gray.
      ,
      • Zhang SP
      • Davis PJ
      • Bandler R.
      Brain stem integration of vocalization: role of nucleus retroambigualis.
      Our assertions neither contradict nor challenge any hypothesis, data, or results published by a wider scientific community on PAG control of vocal behavior, as wrongly claimed by Davis and Zhang. The main result of the work of Davis and Zhang
      • Zhang SP
      • Davis PJ
      • Bandler R
      • et al.
      Brain stem integration of vocalization: role of the midbrain periaqueductal gray.
      is “two fundamental patterns of patterned respiratory and laryngeal muscle activity evoked following stimulation of discrete regions of the PAG,” which, according to them, may or may not be associated with vocalization (page 11346). Zhang et al
      • Zhang SP
      • Davis PJ
      • Bandler R
      • et al.
      Brain stem integration of vocalization: role of the midbrain periaqueductal gray.
      ,
      • Zhang SP
      • Davis PJ
      • Bandler R.
      Brain stem integration of vocalization: role of nucleus retroambigualis.
      did not undertake any specific electromyography (EMG) analysis, except providing integrated waveforms to show patterns of muscle activity. The records they provided are also based on cohorts of D, L, Homocysteic Acid (DLH) injections, as well as muscle recordings obtained in subsets of cats (genioglossus (GG)/digastric (DG) data based on 2 experiments, 11C/IO data based on 4 experiments and so on, as tabulated in their results section). With such subset of recordings for each muscle, normalization of their data across all animals to undertake various quantitative analyses is not possible. That is probably the reason why Zhang et al
      • Zhang SP
      • Davis PJ
      • Bandler R
      • et al.
      Brain stem integration of vocalization: role of the midbrain periaqueductal gray.
      classified vocalizations as type A and type B sounds and mainly focused on elucidation of patterned muscle activity following stimulation of the PAG.
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