Summary
Purpose
The present study was designed to observe the concentration of hydrogen peroxide (H2O2) in exhaled breath condensate (EBC) after induced phonotrauma.
Methods
Thirty-five participants were randomly assigned to one of two conditions (1) Vocal
demand and (2) Control. Participants in the experimental group (vocal demand) were
asked to read aloud some texts during 1 hour, at 85-90 dB. Inflammation (H2O2 from exhaled breath condensate), acoustic, aerodynamic, and subjective measures were
obtained at four time points: before vocal demand (baseline), immediately after baseline,
4-hour after baseline, and 24 hours after baseline. The same acquisition process was
implemented for subjects in control group, except that they were not asked to engage
in any vocal demand tasks at all.
Results
As for biological samples, a significant effect for group was observed. Higher values
were found for participants in experimental condition. Significant differences were
observed for within contrasts in the experimental group, namely 4 hours against baseline,
4 hours against immediately post, and 24 hours against 4 hours. Instrumental outcomes
did not show significant differences across the different conditions at any time points.
Self-reported measures (vocal fatigue and sensation of muscle tension) showed a significant
main effect for group and main effect for condition.
Conclusions
Intense vocal demand causes an increase in the concentration of H2O2 obtained from EBC at four hours after baseline, which is compatible with the generation
of an inflammatory process in the vocal folds (phonotrauma). Moreover, the increase
in the sensation of vocal fatigue and muscle tension after demand tasks seems to be
an immediate reaction that did not match in time with the increment of H2O2 concentration.
Key Words
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Article info
Publication history
Published online: March 26, 2022
Accepted:
February 22,
2022
Publication stage
In Press Corrected ProofFootnotes
This research was supported by grants from ANID (Grants FONDECYT 11180291)
Identification
Copyright
© 2022 The Voice Foundation. Published by Elsevier Inc. All rights reserved.
