Summary
Background/Objectives
While voice-related therapeutic interventions are often researched preclinically in
the porcine model, there are no well-established methods to induce porcine glottic
phonation. Described approaches, such as training animals to phonate for positive
reinforcement are time-consuming and plagued by inherent variability in the type of
phonation produced and contamination of background noise. Thus, a reliable method
of assessing glottic phonation in the porcine model is needed.
Methods
In this study, we have created a novel pulley-based apparatus with harness for “pig-lifting”
with surrounding acoustic insulation and high-directional microphone with digital
recorder for recording phonation. Praat and Matlab were used to analyze all porcine
vocalizations for fundamental frequency (F0), intensity, duration of phonation and
cepstral peak prominence (CPP). Glottic phonation was detected using F0 (≥2000 hz),
duration (≥3 seconds) and researcher perceptual judgment. Partial-glottic phonations
were also analyzed. Reliability between researcher judgment and acoustic measures
for glottic phonation detection was high.
Results
Acoustic analysis demonstrated that glottic and partial-glottic phonation was consistently
elicited, with no formal training of the minipigs required. Glottic vocalizations
increased with multiple lifts. Glottic phonation continued to be elicited after multiple
days but became less frequent. Glottic and partial-glottic phonations had similar
CPP values over the 6 experimental days.
Conclusion
Our cost-effective, reliable method of inducing and recording glottic phonation in
the porcine model may provide a cost effective, preclinical tool in voice research.
Key Words
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Article info
Publication history
Published online: April 30, 2022
Accepted:
February 24,
2022
Publication stage
In Press Corrected ProofFootnotes
Portions of this paper were presented as a poster at the ALA meeting at COSM on April 7, 2021.
This project was supported by award number R01DC014070-01A1 from the National Institute on Deafness and Other Communication Disorders (NIDCD) within the National Institutes of Health (NIH).
Identification
Copyright
© 2022 The Voice Foundation. Published by Elsevier Inc. All rights reserved.
