Summary
Objectives
Vocal fold (VF) scarring is the major cause of voice disorders. Cryotherapy is an
effective anti-scarring therapy for skin lesions. The aim of this study was to explore
the anti-scarring potential of cryotherapy in vocal folds.
Methods
The extracellular matrix (ECM) mRNA expression of cryotherapy on normal VF tissue
and the histologic results of cryotherapy on vocal fold healing were studied. Fifteen
rats were introduced cryotherapy on the normal VF bilaterally and were harvested for
real-time polymerase chain reaction (RT-PCR) analysis for collagen I, collagen III,
TGFβ1, decorin, fibronectin and HAS1 at 1 day, 3 days and 7 days. Ten rats were unilaterally
injured by stripping lamina propria and immediately treated with or without cryotherapy
and were harvested at 2 months for histological and immunohistochemical analysis.
Results
Regenerative effect of cryotherapy was validated of ECM gene expression. Histological
and immunohistochemical analysis showed significantly increased hyaluronan, decreased
collagen, and increased decorin deposition in injury-cryotherapy cohort compared with
injury control cohort and normal control cohort.
Conclusions
Cryotherapy may provide an optimal environment for vocal fold tissue regeneration.
The results of the present investigation suggest that cryotherapy has therapeutic
potential in prevention and treatment of vocal fold scarring.
Key Words
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Journal of VoiceAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
REFERENCES
- Current approaches to management of vocal fold scar.Curr Opin Otolaryngol Head Neck Surg. 2021; 29: 465-472https://doi.org/10.1097/MOO.0000000000000769
- Prevention of vocal fold scarring by local application of basic fibroblast growth factor in a rat vocal fold injury model.Laryngoscope. 2017; 127: E67-E74https://doi.org/10.1002/lary.26138
- Extracellular matrix gene expression during wound healing of the injured rat vocal fold.Otolaryngol Head Neck Surg. 2009; 140: 757-761https://doi.org/10.1016/j.otohns.2008.12.065
- Vocal fold proteoglycans and their influence on biomechanics.Laryngoscope. 1999; 109: 845-854
- Repairing the vibratory vocal fold.Laryngoscope. 2018; 128: 153-159https://doi.org/10.1002/lary.26801
- Vocal fold myofibroblast profile of scarring.Laryngoscope. 2016; 126: E110-E117https://doi.org/10.1002/lary.25581
- Diagnosis and treatment of persistent dysphonia after laryngeal surgery: a retrospective analysis of 62 patients.Laryngoscope. 1994; 104: 1084-1091https://doi.org/10.1288/00005537-199409000-00007
- The unsolved chapter of vocal fold scars and how tissue engineering could help us solve the problem.Eur Arch Otorhinolaryngol. 2016; 273: 2279-2284https://doi.org/10.1007/s00405-015-3668-8
- Different types of Auricular Keloids and treatment by intralesional Cryosurgery: best practice for obtaining long-lasting clinical results.Dermatology. 2022; 238: 170-179https://doi.org/10.1159/000514954
- Cryotherapy modifies synthetic activity and differentiation of keloidal fibroblasts in vitro.Exp Dermatol. 2003; 12: 673-681https://doi.org/10.1034/j.1600-0625.2003.00015.x
- Identification of distinct layers within the stratified squamous epithelium of the adult human true vocal fold.Laryngoscope. 2015; 125: E313-E319https://doi.org/10.1002/lary.25264
- The use of the pulse dye laser in the treatment of vocal fold scar: a preliminary study.Laryngoscope. 2008; 118: 1884-1888https://doi.org/10.1097/MLG.0b013e31817d7546
- Efficacy of treating children with anterior commissure and true vocal fold respiratory papilloma with the 585-nm pulsed-dye laser.Arch Otolaryngol Head Neck Surg. 2007; 133: 127-130https://doi.org/10.1001/archotol.133.2.127
- A preliminary case report of a high-quality cost-effective rigid laryngoscopy setup.Ann Otol Rhinol Laryngol. 2017; 126: 411-414https://doi.org/10.1177/0003489417693863
- Vibratory function and healing outcomes after small intestinal submucosa biomaterial implantation for chronic vocal fold scar.Laryngoscope. 2018; 128: 901-908https://doi.org/10.1002/lary.26883
- Interstitial protein alterations in rabbit vocal fold with scar.J Voice. 2003; 17: 377-383
- Fibroblasts in human vocal fold mucosa.Acta Otolaryngol. 1999; 119: 271-276
- Primed fibroblasts and exogenous decorin: potential treatments for subacute vocal fold scar.Otolaryngol Head Neck Surg. 2006; 135: 937-945https://doi.org/10.1016/j.otohns.2006.07.011
- Vocal fold healing after laser cordectomy with adjuvant cryotherapy.Laryngoscope. 2006; 116: 1580-1584https://doi.org/10.1097/01.mlg.0000231738.80952.7c
- Cryotherapy modifies synthetic activity and differentiation of keloidal fibroblasts in vitro.Exp Dermatol. 2003; 12: 673-681
- Intralesional cryosurgery enhances the involution of recalcitrant auricular keloids: a new clinical approach supported by experimental studies.Wound Repair Regen. 2006; 14: 18-27https://doi.org/10.1111/j.1743-6109.2005.00084.x
- Histologic and rheologic characterization of vocal fold scarring.J Voice. 2002; 16: 96-104
- Effect of exogenous decorin on cell morphology and attachment of decorin-deficient fibroblasts.J Biochem. 1996; 119: 743-748
- Model structure of decorin and implications for collagen fibrillogenesis.J Biol Chem. 1996; 271: 31767-31770
- Small leucine-rich proteoglycans, decorin and fibromodulin, are reduced in postburn hypertrophic scar.Wound Repair Regen. 2011; 19: 368-378https://doi.org/10.1111/j.1524-475X.2011.00677.x
- Morphological and extracellular matrix changes following vocal fold injury in mice.Cells Tissues Organs. 2010; 192: 262-271https://doi.org/10.1159/000315476
- Recombinant human decorin inhibits cell proliferation and downregulates TGF-beta1 production in hypertrophic scar fibroblasts.Burns. 2007; 33: 634-641https://doi.org/10.1016/j.burns.2006.08.018
- Fibrillogenesis of collagen types I, II, and III with small leucine-rich proteoglycans decorin and biglycan.Biomacromolecules. 2006; 7: 2388-2393https://doi.org/10.1021/bm0603746
- Fibronectin and adhesion molecules on canine scarred vocal folds.Laryngoscope. 2003; 113: 966-972https://doi.org/10.1097/00005537-200306000-00010
- Fibroblasts from post-burn hypertrophic scar tissue synthesize less decorin than normal dermal fibroblasts.Clin Sci (Lond). 1998; 94: 541-547
- Histologic characterization of rat vocal fold scarring.Ann Otol Rhinol Laryngol. 2005; 114: 183-191https://doi.org/10.1177/000348940511400303
Article info
Publication history
Published online: September 28, 2022
Accepted:
August 30,
2022
Publication stage
In Press Corrected ProofFootnotes
Ting Gong and Pengcheng Yu contributed to the work equally and should be regarded as co-first authors.
The work is supported by the Program of Shanghai Academic Research Leader (Grant No. 19XD1403600).
Identification
Copyright
© 2022 The Voice Foundation. Published by Elsevier Inc. All rights reserved.
