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Cryotherapy Modifies Extracellular Matrix Expression of Vocal Fold in Rat Models

  • Ting Gong
    Affiliations
    School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China
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  • Pengcheng Yu
    Affiliations
    Department of Otolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China
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  • Tao Lu
    Affiliations
    Shanghai University of Traditional Chinese Medicine, Shanghai, China
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  • Jiwei Chen
    Affiliations
    Shanghai University of Traditional Chinese Medicine, Shanghai, China
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  • Jinxia Mi
    Affiliations
    Shanghai University of Traditional Chinese Medicine, Shanghai, China
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  • Rui Fang
    Correspondence
    Address correspondence and reprint requests to Rui Fang, The Department of Otolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai 200030, China.
    Affiliations
    Department of Otolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and Throat Hospital of Fudan University, Shanghai, China
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  • Chunlei Shan
    Correspondence
    Address correspondence and reprint requests to Chunlei Shan, School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China.
    Affiliations
    School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China

    Center of Rehabilitation Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China

    Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation, Ministry of Education, Shanghai, China
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Published:September 28, 2022DOI:https://doi.org/10.1016/j.jvoice.2022.08.031

      Summary

      Objectives

      Vocal fold (VF) scarring is the major cause of voice disorders. Cryotherapy is an effective anti-scarring therapy for skin lesions. The aim of this study was to explore the anti-scarring potential of cryotherapy in vocal folds.

      Methods

      The extracellular matrix (ECM) mRNA expression of cryotherapy on normal VF tissue and the histologic results of cryotherapy on vocal fold healing were studied. Fifteen rats were introduced cryotherapy on the normal VF bilaterally and were harvested for real-time polymerase chain reaction (RT-PCR) analysis for collagen I, collagen III, TGFβ1, decorin, fibronectin and HAS1 at 1 day, 3 days and 7 days. Ten rats were unilaterally injured by stripping lamina propria and immediately treated with or without cryotherapy and were harvested at 2 months for histological and immunohistochemical analysis.

      Results

      Regenerative effect of cryotherapy was validated of ECM gene expression. Histological and immunohistochemical analysis showed significantly increased hyaluronan, decreased collagen, and increased decorin deposition in injury-cryotherapy cohort compared with injury control cohort and normal control cohort.

      Conclusions

      Cryotherapy may provide an optimal environment for vocal fold tissue regeneration. The results of the present investigation suggest that cryotherapy has therapeutic potential in prevention and treatment of vocal fold scarring.

      Key Words

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