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Changes of Pepsin Concentration in Saliva Sample According to Storage Period

  • Author Footnotes
    1 Hee Jin Kang and Joo Kyung Noh have contributed equally to this study and should be considered co-first authors.
    Hee Jin Kang
    Footnotes
    1 Hee Jin Kang and Joo Kyung Noh have contributed equally to this study and should be considered co-first authors.
    Affiliations
    Department of Otolaryngology Head & Neck Surgery, Kyung Hee University School of Medicine, Kyung Hee University Medical Center, Seoul, Republic of Korea
    Search for articles by this author
  • Author Footnotes
    1 Hee Jin Kang and Joo Kyung Noh have contributed equally to this study and should be considered co-first authors.
    Joo Kyung Noh
    Footnotes
    1 Hee Jin Kang and Joo Kyung Noh have contributed equally to this study and should be considered co-first authors.
    Affiliations
    Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul, Republic of Korea
    Search for articles by this author
  • Min Kyeong Lee
    Affiliations
    Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul, Republic of Korea
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  • Seon Rang Woo
    Affiliations
    Department of Otolaryngology Head & Neck Surgery, Kyung Hee University School of Medicine, Kyung Hee University Medical Center, Seoul, Republic of Korea

    Central Laboratory, Medical Science Research Institute, Kyung Hee University Medical Center, Seoul, Republic of Korea
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  • Jung Min Park
    Affiliations
    Department of Otolaryngology Head & Neck Surgery, Kyung Hee University School of Medicine, Kyung Hee University Medical Center, Seoul, Republic of Korea
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  • Young Chan Lee
    Affiliations
    Department of Otolaryngology Head & Neck Surgery, Kyung Hee University School of Medicine, Kyung Hee University Medical Center, Seoul, Republic of Korea
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  • Seong-Gyu Ko
    Affiliations
    Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea
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  • Young-Gyu Eun
    Correspondence
    Address correspondence and reprint requests to Young Gyu Eun, Department of Otolaryngology Head & Neck Surgery, Kyung Hee University Medical Center, #1 Hoegi-dong, Dongdaemun-gu, Seoul 02447, Republic of Korea.
    Affiliations
    Department of Otolaryngology Head & Neck Surgery, Kyung Hee University School of Medicine, Kyung Hee University Medical Center, Seoul, Republic of Korea

    Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul, Republic of Korea
    Search for articles by this author
  • Author Footnotes
    1 Hee Jin Kang and Joo Kyung Noh have contributed equally to this study and should be considered co-first authors.
Published:December 30, 2022DOI:https://doi.org/10.1016/j.jvoice.2022.12.010

      Summary

      Objectives

      To determine whether the concentration of pepsin in the saliva sample changes according to the storage period of the sample.

      Methods

      Forty eight patients with suspected laryngopharyngeal reflux were included in this study. Saliva samples were collected from each patient and each sample divided into six and stored for different period of time. Pepsin concentration was measured using enzyme-linked immunosorbent assay. A comparison was made between the pepsin concentration measured immediately and the concentration measured after storage for 1 week, 2 weeks, 1 month, 3 months, and 6 months.

      Results

      No significant difference in pepsin concentrations were detected between the sample analyzed immediately and those analyzed at 1 week, 2 weeks, 1 month, and 3 months after saliva collection. A significant difference in pepsin concentration was observed in the sample analyzed immediately and the sample analyzed 6 months after saliva collection.

      Conclusions

      Pepsin concentration in saliva samples did not demonstrate a significant difference between the concentration measured immediately and the concentration measured 3 months after saliva collection, although a significant difference was observed in the concentration measured 6 months after collection.

      Key Words

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