Summary
Objectives
To determine whether the concentration of pepsin in the saliva sample changes according
to the storage period of the sample.
Methods
Forty eight patients with suspected laryngopharyngeal reflux were included in this
study. Saliva samples were collected from each patient and each sample divided into
six and stored for different period of time. Pepsin concentration was measured using
enzyme-linked immunosorbent assay. A comparison was made between the pepsin concentration
measured immediately and the concentration measured after storage for 1 week, 2 weeks,
1 month, 3 months, and 6 months.
Results
No significant difference in pepsin concentrations were detected between the sample
analyzed immediately and those analyzed at 1 week, 2 weeks, 1 month, and 3 months
after saliva collection. A significant difference in pepsin concentration was observed
in the sample analyzed immediately and the sample analyzed 6 months after saliva collection.
Conclusions
Pepsin concentration in saliva samples did not demonstrate a significant difference
between the concentration measured immediately and the concentration measured 3 months
after saliva collection, although a significant difference was observed in the concentration
measured 6 months after collection.
Key Words
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Article info
Publication history
Published online: December 30, 2022
Accepted:
December 14,
2022
Publication stage
In Press Corrected ProofFootnotes
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIT) (No. 2020R1A5A2019413) and by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (No. HI20C1205).
Identification
Copyright
© 2022 The Voice Foundation. Published by Elsevier Inc. All rights reserved.
