We assessed the safety of flexible fiber-based lasers including the potassium-titanyl-phosphate (KTP), CO2, and Holmium lasers using a rabbit vocal fold model and provided the safety evidence of laser before human clinical trial.
In all, 120 male New Zealand white rabbits were used. Each laser was used to induce acute and chronic vocal fold injuries in 40 rabbits. The same laser energy with the same intensity and frequency were used in all cases, and we evaluated outcomes via surface scanning electron microscopy (SEM) and histological examination 1 day after injury. Histological and high-speed vocal fold vibration examinations were evaluated 1 month after injury. Surface injury roughness grading was done via SEM, and the Acute injury ratio and lamina propria (LP) ratio were also calculated. The dynamic glottal gap was measured through functional analyses based on recordings from a high-speed digital camera.
The Holmium laser caused significantly more vocal fold damage than did the KTP and CO2 lasers as revealed by SEM and evaluation of acute and chronic injuries. Functional analysis with high-speed digital camera indicated that the holmium laser reduced dynamic glottal gap compared with the normal vocal fold, where the other lasers did not.
The histological and functional analyses of rabbit vocal fold experiments indicated that fiber-based laryngeal laser surgery for vocal fold lesions could be performed relatively safely using a KTP or CO2 laser.
Abbreviations:FLS (fiber-based laryngeal laser surgery), KTP (potassium-titanyl-phosphorous), SEM (scanning electron microscopy), LP (lamina propria)
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Published online: February 25, 2023
Accepted: January 23, 2023
Publication stageIn Press Corrected Proof
This study was presented at the 92nd Annual Congress of Korean Society of Otorhinolaryngology-Head and Neck Surgery, 2018 Spring Meeting of Korean Association of Otorhinolaryngologists in conjunction with International Congress of ORL-HNS 2018 in Seoul, Republic of Korea at April 29, 2018.
© 2023 The Voice Foundation. Published by Elsevier Inc. All rights reserved.