Summary
Objectives
The purpose of this research is to determine which type of humid air should be suggested
for vocal hygiene by demonstrating the effects of hot humid air and cold humid air
on the vocal cord mucosa using different histological methods.
Study Design
Randomized controlled study.
Methods
Cold or hot humid air was applied to the rats for 30 minutes/day for 10 days using
a humid air machine placed in a closed glass cage. The control group did not receive
any treatment and were kept in their cages under normal laboratory conditions. The
animals were sacrificed and their larynxes were removed on the 11th day. Histologically,
lamina propria (LP) thickness was measured by Crossman's three stain and the number
of mast cells in 1 square millimeter of lamina propria was measured by toluidine blue.
In immunohistochemical staining, the intensity of zonula occludens-1 (ZO-1) staining
was measured using a rabbit polyclonal antibody and scored from 0 (no staining) to
3 (intense staining). One-way ANOVA and Kruskal-Wallis tests were used to compare
groups.
Results
The mean LP thickness was thinner in rats exposed to cold humid air (CHA) than in
the control group (P = 0.012). In terms of LP thickness, other intergroup comparisons (cold vs hot and
control vs hot) showed no statistically significant difference between groups (P > 0.05). The mean mast cell count did not differ between groups. The hot humid air
(HHA) group had more intense ZO-1 staining than the other groups (P < 0.001). There was no difference in ZO-1 staining intensity between the control
group and CHA group.
Conclusion
HHA and CHA administration had no negative effects on inflammatory findings in the
vocal cords (mast cell count or LP thickness). While HHA appears to strengthen the
epithelial barrier (denser ZO-1 staining), the physiologic outcomes, such as bronchoconstriction,
should be cautiously assessed.
Key words
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Article info
Publication history
Published online: March 28, 2023
Accepted:
February 20,
2023
Publication stage
In Press Corrected ProofFootnotes
This study was conducted at Ondokuz Mayıs University Experimental Animal Research Center.
The authors declared that this study has received no financial support.
The findings of this study were presented as an e-poster at the 13th Congress of the European Laryngological Society (Digital: May 28, 2021).
Identification
Copyright
© 2023 The Voice Foundation. Published by Elsevier Inc. All rights reserved.
